Establishment of Agrobacterium-mediated genetic transformation system in Dahlia
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چکیده
منابع مشابه
Agrobacterium-Mediated Genetic Transformation of Switchgrass
and the bar gene was transmitted through both male and female gametes and expressed in T1 progeny. Although Agrobacterium tumefaciens has been successfully used Microprojectile bombardment and gene transfer by to transfer genes to a wide range of plant species, it has received litA. tumefaciens are currently the two most common tle attention for transformation of forage grasses. Therefore, the ...
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Agrobacterium tumefaciens is a Gram-negative soil phytopathogenic bacterium that causes the crown gall disease of dicotyledonous plants, which is characterized by a tumorous phenotype. It induces the tumor by transferring a segment of its Ti plasmid DNA (transferred DNA, or T-DNA) into the host genome and genetically transforming the host. One century has past after A. tumefaciens was firstly i...
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Background: Oat (Avena sativa) with high nutritive value and fiber content is used as the main food grain in many countries for human diet as well as animal feed. Recently, it became difficult to transfer new genes through the conventional breeding due to the lack of desirable traits. Objectives: The current study aimed at achieving a st...
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To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
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Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2013
ISSN: 1342-4580,1347-6114
DOI: 10.5511/plantbiotechnology.13.0128a